|
KMID : 0380020030180010001
|
|
Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 1 p.1 ~ p.7
|
|
|
Enantioselective Hydrolysis for the Precursor of Azole-containing Compounds using Acinetobacter sp. SY-01 Lipase and increase of Enantioselectivity by the Removal of Reaction Products
|
|
Yun Moon-Young
Shin Pyong-Gyun
Chung Chan-Sung
Park Jung-Keug
|
|
|
Abstract
|
|
|
|
Screening of a strain was carried out to produce an enantioselective lipase toward the precursor of ltraconazole as azole-containg compounds, which are well known as antifungal drug agents. An Acinetobacter sp. SY-01 strain which can selectively hydrolyze the racemic substrates was isolated and the racemic substrate was resolved to the S-ester in 95.6% enantiomeric excess after 74.8% hydrolysis. The optimum temperature and pH for the conversion were , pH 7.0. However, the temperature and pH had no effect on the enantiomeric excess. Addition of solvents decreased the conversion and slightly increased the enantiomeric excess. However, the kind of solvents had no effect on enantiomeric excess. The substrate concentration decrease enantiomeric excess and this is confirmed by the products generated from hydrolysis, and also enantiomeric excess could be increased by the removal of reaction products.
|
|
|
KEYWORD
|
|
|
Screening, lipase, acinetobacter, azole-containing compounds, enantioselective hydrolysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|